35.6 mg ferrous ammonium sulfate hexahydrate-acidify with 6 M H₂SO₄

36 mg iron (III) nitrate, dissolve in dilute nitric acid (5-mL 6 M HNO₃/L)

Initial: Sodium acetate 0.1 M solution, adjust to pH 4.5 with 0.1 M acetic acid. Make up Hydroxylamine solution using adjusted sodium acetate solution. Adjust final pH of Hydroxylamine solution to pH 4.7 using 6 M NaOH

356 mg / L Ferrous ammonium sulfate hexahydrate-acidify with 6 M H₂SO₄

FerroZine solution (1.0g/L)

1,10-phenanthroline solution 1.0 g / L

terpyridine solution (dissolve in ethanol) 0.500 g / L

0.005 M Citrate (Sodium Citrate 1.47 g / L)

0.005 M Phosphate (Use Phosphoric acid solid 0.50 g / L )

0.005 M Ammonium (Ammonium Chloride 0.27 g / L)

Combine all solids and dilute to 1 L with H₂O

Adjust pH to 1.5 using concentrated HCl and pH meter

Bromothymol Blue 0.1 g in 16-mL 0.01 N NaOH + 234-mL H₂O

Thymolphthalein 0.1 g in 16-mL 0.01 N NaOH + 234-mL H₂O

Bromocresol Purple 0.1 g in 16-mL 0.01 N NaOH + 234-mL H₂O

Dissolve 11.8 g succinic acid + 2.7 g Brij 35 in 800-mL of H₂O. Warm slightly to dissolve Brij 35 if necessary. Adjust pH to 4.0 with NaOH (dropwise). Dilute to 1-L volume with H₂O. Store in refrigerator.

Dissolve 419 mg bromocresol green in 10 mL of 0.1 M NaOH. Dissolve completely and bring to 1-L volume with H₂O.

1 volume of bromocresol green solution/3 volumes of succinate buffer. Adjust pH to 4.2 ± 0.05 pH units. Label: Bromocresol green working solution, pH 4.2; store in refrigerator.

Dissolve 9g of NaCl in 900 mL of H₂O. Bring to 1-L volume with H₂O.

Dissolve 2.50 g of Albumin in 800 mL NaCl 9g/L solution. Dissolve completely and bring to 1-L volume with NaCl 9g/L solution. Label: Albumin 0.250 g/dL in 9g/L NaCl solution ;store in refrigerator

Each student will need approximately 125 mL of 1 HCl and 125 mL H₂SO₄. Unknown range from 0.075—0.110 M. Label unknown bottles with letter codes and a label instructing students to take only 125 mL of the assigned unknown.

Dissolve 108.80 g potassium dihydrogen phosphate in 2 L of deionized H₂O. After dissolving, insert pH probe and adjust pH to 6.50 with 2 M KOH. After adjustment, bring solution to 4-L mark with H₂O.

Dissolve 217.60 g potassium dihydrogen phosphate in 2 L of deionized H₂O. After dissolving, insert pH probe and adjust pH to 6.50 with 2 M KOH. After adjustment, bring solution to 4-L mark with H₂O.

Dissolve 0.362 g 4-nitrophenyl acetate in 3.5 L H₂O. Bring to 4 L.

Imidazole, 25-mM concentration

Dissolve 6.80 g Imidazole in 3.5 L H₂O. Bring to 4 L

2-Methylimidazole, 25-mM concentration

4-Methylimidazole, 25-mM concentration

NaOH: ~0.040 M, adjust to pH 11.10—11.30

NaOH: ~0.040 M, adjust to pH 11.70—12.0

Fe(NH₄)₂(SO₄)₂•6H₂O: approx. 300 g/L, then add 3 M sulfuric acid until acidic

1 digital multimeter w/ alligator clips

1 chem cell plastic

1 small piece of silver wire

250 g/L Cu(SO₄)₂•5H₂O, add sulfuric acid to acidify solution.

Catalase source: Sigma Chemicals, product No. C-9322

Catalase from bovine liver, 3260 units/mg solid

Concentration of catalase solution is determined by experimentation. The age of the catalase solid or solution determines how concentrated the solution must be. Start with a 0.5 g catalase / 100-mL solution concentration and test the reaction using 4 drops of catalase in 75 mL of 5% H₂O₂. The reaction should proceed rapidly with swirling, but with a minimum amount of foaming. Adjust the concentration up if the reaction does not come to completion in 2—4 minutes.

These can be made as "concrete ice cubes," using concrete mix from a hardware store. Mix this in a bowl and fill an ice cube tray with the wet mixture. Spray the ice cube tray with a silicone spray before filling the tray with the concrete mix. The pieces of concrete come out easily, just like ice from a tray.

Silver nitrate, 0.1 M

Sodium chloride, 0.1 M

Sodium phosphate, 0.1 M

Sodium nitrate, 0.1 M

Sodium carbonate, 0.1 M

Sodium sulfate, 0.1 M

Zinc sulfate, 0.1 M

Use white coral sand and powdered sodium phosphate. Various mixtures of anhydrous sodium phosphate and sand containing 15—75% sodium phosphate by mass. The key to the unknowns should report the phosphorus present as P₂O₅, because that is how students are to report their results and it should include the percent sand by mass. Students will obtain this information from the lab instructor after they complete the experiment.

TEA (triethanolamine) analytical grade, Brij-35

(solution TEA: 20% v/v aqueous solution, containing 80 microliters of 10% Brij-35 solution)

Tubes are loaded with 40 microliters of mesh solution each

2% w/v aqueous solution of sulfanilamide in 6% v/v phosphoric acid.

Prepare 250-mL of aqueous solution containing 5 g of sulfanilamide and 15-mL of concentrated phosphoric acid)

0.14% w/w aqueous solution of NEDA

Prepare 250-mL of aqueous solution containing 0.35 g NEDA

(N-1-naphthylethylene-diamine dihydrochloride)

Cut to small squares and pushed to the bottom of analysis tubes with a dowel.

Dilute to 18 L with water in a 20-L carboy. Add 0.099 g of bromocresol green sodium salt and 0.066 g of methy red sodium salt; dissolve. Bring to 20- L volume with water. Adjust pH to 4.8—5.0 using sodium hydroxide pellets.