Metabolism Integrated into the Coverage of Diet and Obesity
New information about the role of leptins in hunger and satiety has greatly influenced how we think about the growing epidemics of obesity and diabetes. In this edition, we cover the integration of metabolism in the context of diet and obesity.
Expanded, Two-Chapter Coverage of Gene Regulation:
Chapter 31, The Control of Gene Expression in Prokaryotes
Chapter 32, The Control of Gene Expression in Eukaryotes
The new edition’s increased coverage of the biochemical aspect of eukaryotic gene regulation reflects the dramatic growth of information in this area. The new chapters address recent discoveries such as quorum sensing in prokaryotes, induced pluripotent stem cells and the role of microRNAs in regulating gene expression.
Updated Presentation of Experimental Techniques in
Chapter 3, Exploring Proteins and Proteomes
Chapter 5, Exploring Genes and Genomes
Chapter 6, Exploring Evolution and Bioinformatics
These chapters give students a more contemporary, more practical understanding of the techniques that they will be using in the lab. This edition includes expanded explanations of mass spectrometry and X-ray crystallography, as well as coverage of new techniques like next-generation sequencing and real-time PCR in the context of their importance to modern biochemical research.
The Latest Research Breakthroughs
Exciting advances and new topics in the new edition include:
• Osteogenesis imperfecta, or brittle bone disease (Chapter 2)
• Intrinsically unstructured proteins and metamorphic proteins (Chapter 2)
• Recent updates in protein misfolding diseases (Chapter 2)
• The use of recombinant DNA technology in protein purification (Chapter 3)
• Expanded discussion of mass spectrometry and X-ray crystallography (Chapter 3)
• Next generation sequencing methods (Chapter 5)
• Real-time PCR (Chapter 5)
• DNA microarrays (Chapter 5)
• Carbon monoxide poisoning (Chapter 7)
• Single-molecule studies of enzyme kinetics (Chapter 8)
• Myosins as a model of a catalytic strategy for ATP hydrolysis (Chapter 9)
• Glycobiology and glycomics (Chapter 11)
• Hurler disease (Chapter 11)
• Avian influenza H5N1 (Chapter 11)
• Lipid rafts (Chapter 12)
• Transferrin as an example of receptor-mediated endocytosis (Chapter 12)
• Long QT syndrome and arrhythmia caused by inhibiting potassium channels (Chapter 13)
• Defects in the citric acid cycle and cancer (Chapter 17)
• Synthesizing a more efficient rubisco (Chapter 20)
• The structure of mammalian fatty acid synthetase (Chapter 22)
• Pyrimidine salvage pathways (Chapter 25)
• Physical association of enzymes in metabolic pathways (Chapter 25)
• Phosphatidic acid phosphatase in the regulation of lipid metabolism (Chapter 26)
• Regulation of SCAP-SREBP movement in cholesterol metabolism (Chapter 26)
• Mutations in the LDL receptor (Chapter 26)
• The role of HDL in protecting against arteriosclerosis (Chapter 26)
• Aromatase inhibitors in the treatment of breast and ovarian cancer (Chapter 26)
• The role of leptin in long-term caloric homeostasis (Chapter 27)
• Obesity and diabetes (Chapter 27)
• Exercise and its effects on cellular biochemistry (Chapter 27)
• Updated detailed mechanism of helicase’s action (Chapter 28)
• Updated detailed mechanism of topoisomerase’s action (Chapter 28)
• Riboswitches (Chapter 29)
• Production of small regulatory RNAs (Chapter 29)
• Vanishing white matter disease (Chapter 30)
• Quorum sensing (Chapter 31)
• Biofilms (Chapter 31)
• Induced pluripotent stem cells (Chapter 32)
• The role of microRNAs in gene regulation (Chapter 32)
• How vaccines work (Chapter 34)
• Structure of myosin head domains (Chapter 35)
Twice as Many End-of-Chapter Problems of a variety of types:
• Traditional problems test biochemical knowledge and the ability to use this knowledge.
• Mechanism problems ask students to suggest or elaborate a chemical mechanism.
• Data interpretation problems ask questions about a set of data provided in tabulated or graphic form. These problems give students a sense of how scientific conclusions are reached.
• Chapter integration problems require students to use information from several chapters to reach a solution.
New Molecular Evolution features:
• Why only L amino acids comprise proteins
• Evolutionary relationships between ion pumps
• The coordination of the Calvin cycle and the pentose phosphate pathway
• Evolution of glycogen phosphorylase
• Sigma factors in bacterial transcription
• miRNAs in gene evolution
New Clinical Applications features:
• Osteogenesis imperfecta
• Protein misfolding diseases
• Carbon monoxide poisoning
• Hurler disease
• Long QT syndrome
• Defects in the citric acid cycle and cancer
• The role of HDL in protecting against arteriosclerosis
• Aromatase inhibitors in the treatment of breast and ovarian cancer
• Vanishing white matter disease
• Vaccines